Choosing between a day 5 vs. day 3 embryo transfer

Embryo transfer is a crucial step in the IVF journey, and one important decision that patients often face is whether to opt for a day 3 or a day 5 embryo transfer. This choice can have implications for implantation success and pregnancy outcomes.

In this blog, we’ll guide you through:

The basics of embryo development.
What happens on day 3 vs. day 5.
Advantages and limitations of each option.
Who is better suited for which approach.
Our expert recommendation approach.

What are day 3 and day 5 embryo transfers?

Day 3 embryos, also called cleavage-stage embryos, are transferred around 72 hours after fertilization. At this stage, the embryo is typically divided into 6–8 cells.
Day 5 embryos, also known as blastocysts, are transferred 120 hours (5 days) after fertilization. By day 5, the embryo has developed a fluid-filled cavity and differentiated into an inner cell mass and outer trophectoderm.

Advantages of Day 3 embryo transfer

Suitable for low embryo numbers: Ideal for patients who produce fewer embryos, as waiting until day 5 might risk losing viable embryos that could have implanted if transferred earlier.

Less time in the lab: Reduces the duration embryos need to survive outside the uterus, which may benefit embryos that don’t thrive in artificial conditions.

Earlier transfer: Allows an earlier chance of implantation for time-sensitive or urgent cycles (e.g., fertility preservation or advancing maternal age).

Advantages of day 5 embryo transfer

Better embryo selection: Embryos that survive until day 5 have shown better developmental potential and are more likely to result in pregnancy.

Higher implantation rates: Blastocysts are closer to the natural timing of implantation, often leading to higher success rates.

Facilitates PGT (Preimplantation Genetic Testing): More accurate genetic testing can be performed at the blastocyst stage with minimal impact on the embryo.

Reduces multiple pregnancies: Fewer embryos are transferred due to higher implantation potential, helping reduce the risk of twins or triplets.

Who might benefit from day 3 transfer?

Women with low ovarian reserve or few embryos.
Patients with previous cycle failures on day 5.
Cases with concerns about in vitro embryo culture conditions.
Those needing urgent embryo transfer (e.g., in special medical conditions).

Who might benefit from day 5 transfer?

Women under 35 with good ovarian response.
Patients with multiple high-quality embryos.
Couples planning for PGT-A or PGT-M.
Those wanting to freeze embryos for later transfer.
Patients seeking single embryo transfer with higher success.

Is one better than the other?

Not necessarily. While day 5 transfers are generally associated with higher success rates, the right choice depends on the individual fertility profile, embryo quality, age, and treatment goals. In some cases, transferring a good-quality day 3 embryo may give you the best chance rather than risking the embryo not reaching day 5.

At our clinic, we don’t use a one-size-fits-all approach. Instead, we evaluate each case on:

Embryo number and quality.
Ovarian response.
History of previous IVF attempts.
Need for genetic testing.
Uterine and endometrial factors.

We make a personalized plan that maximizes your chances of success.

Conclusion

Whether you opt for a day 3 or day 5 embryo transfer, the ultimate goal remains the same — achieving a healthy pregnancy and welcoming your baby into the world. Each approach has its advantages, and the right choice depends on your unique fertility profile, medical history, and emotional readiness. At the heart of it all is a decision that must feel right for you, supported by accurate information and expert care.

At Bavishi Fertility Institute, we understand that every fertility journey is deeply personal. That’s why we take the time to explain every option in detail, guiding you with evidence-based advice, advanced technology, and heartfelt compassion. Our experienced team is committed to helping you make informed choices that give you the best possible chance of success.Book a consultation with Bavishi Fertility Institute today and let us walk with you on your journey to parenthood — with science, support, and sincerity.

Author bio

Dr. Parth Bavishi

Dr. Parth Bavishi, MD in Obstetrics and Gynecology, brings over 12 years of invaluable work experience to his role as Director of Bavishi Fertility Institute, leading a group of IVF clinics committed to helping couples realise their dreams of parenthood.

Bavishi Fertility Institute is dedicated to providing customised and personalised treatments which are simple, safe , smart and successful. Bavishi Fertility Institute works with success and satisfaction for all at heart. Providing an ideal blend of professional treatment and personalised care.

Dr Parth had special training in infertility at Bavishi fertility Institute, the Diamond Institute, USA, and the HART Institute, Japan.

Dr. Bavishi is a distinguished expert in his field. In addition to his clinical practice, Dr. Bavishi is the author of the acclaimed book, ‘Your Miracle in Making: A Couple’s Guide to Pregnancy,’ offering invaluable insights to couples navigating the complexities of fertility. He loves to empower patients to make the correct choice by education both online and offline.

His exceptional contributions have earned him the prestigious Rose of Paracelsus award from the European Medical Association. Dr Parth has been an invited faculty at many national and international conferences.

Beyond his professional endeavors, Dr. Bavishi is an avid traveller who finds solace in exploring new destinations. He also enjoys engaging his mind with brainy puzzles, always seeking new challenges and solutions outside of the medical realm.

FAQs

Can I choose whether I want a Day 3 or Day 5 embryo transfer, or is it solely up to the doctor?

Patients can express their preferences, but the final decision is made collaboratively with the fertility specialist based on clinical factors and embryonic development.

Is there a difference in the cost between Day 3 and Day 5 embryo transfers?

Yes, Day 5 transfers may involve higher costs due to extended laboratory culture and advanced monitoring, especially if blastocyst culture or genetic testing is performed.

Does a Day 5 transfer reduce the chances of needing multiple IVF cycles?

In many cases, yes. Due to higher implantation potential, a Day 5 transfer may lead to pregnancy sooner, reducing the need for repeated cycles.

How do lab conditions impact the success of Day 5 embryo development?

Extended culture to Day 5 requires optimal lab conditions, including strict temperature, pH, and humidity controls to mimic the uterine environment.

What happens to embryos that don’t make it to Day 5?

Embryos that stop developing before Day 5 are typically not viable for transfer or freezing and are usually discarded after consultation with the patient.

Can both Day 3 and Day 5 embryos be frozen for future use?

Yes, both types can be cryopreserved, although blastocysts (Day 5) tend to have higher survival rates post-thaw due to better developmental maturity.

Do Day 5 embryos have a higher chance of resulting in a baby with a higher birth weight or better health outcomes?

Current research does not conclusively show significant differences in long-term health or birth weight between Day 3 and Day 5 transfers.