Male Fertility · WHO 2021 Reference Values

Semen Analysis Calculator

Understand your semen analysis results and what they mean.
Free Tool Instant Results No Data Stored
6
Parameters analysed
WHO 2021
Latest reference values
TMSC
Total motile count calculated
50%
Infertility cases involve male factor

Understanding Your Semen Analysis

Each parameter tells a different story about male fertility. Here's what each one means.

Volume & pH

Normal volume is ≥ 1.4 mL. Low volume may indicate retrograde ejaculation or blocked ejaculatory ducts. pH (7.2–8.0) reflects secretion from seminal vesicles and prostate.

Count & Concentration

Concentration ≥ 16 million/mL and total count ≥ 39 million per ejaculate are normal. Oligozoospermia (low count) has many treatable causes including hormonal imbalance and varicocele.

Motility

At least 42% of sperm should be moving (total motility) and 30% should move progressively forward. Poor motility (asthenozoospermia) reduces the chance of natural fertilisation.

Morphology

At least 4% normal forms (Kruger strict criteria) are required. Morphology reflects sperm DNA packaging quality. Teratozoospermia can affect fertilisation even with ICSI.

DNA & WBC

WBCs > 1 million/mL (leukocytospermia) may indicate infection or inflammation. Sperm DNA fragmentation (not in this calculator) is a separate test for cases with normal parameters but poor outcomes.

Treatment Path

Results guide treatment: Mild issues may improve with lifestyle and supplements. Moderate issues often respond to medication. Severe cases may need ICSI, surgical retrieval, or donor sperm.

WHO 2021 Reference Values at a Glance

The 5th centile values from the WHO 2021 manual — the lowest normal values from fertile men.

ParameterLower Reference LimitClinical Condition if BelowImpact
Volume≥ 1.4 mLHypospermiaFewer sperm delivered to cervix
pH7.2 – 8.0Abnormal secretionsReflects seminal vesicle / prostate function
Sperm Concentration≥ 16 M/mLOligozoospermiaReduced chance of natural conception
Total Sperm Count≥ 39 M/ejaculateOligozoospermiaLow absolute number of sperm
Total Motility (PR + NP)≥ 42%AsthenozoospermiaFewer sperm capable of reaching egg
Progressive Motility≥ 30%AsthenozoospermiaReduced forward movement efficiency
Morphology (Kruger)≥ 4% normalTeratozoospermiaAffects fertilisation ability
WBC / Leucocytes< 1 M/mLLeukocytospermiaSuggests infection or inflammation
TMSC≥ 9 M (IUI)Poor IUI candidate if lowerKey metric for IUI suitability

Source: WHO Laboratory Manual for the Examination and Processing of Human Semen, 6th Edition (2021)

Enter Your Semen Analysis Values

Enter the values from your report. You may leave any field blank if not available.

mL

WHO ref: ≥ 1.4 mL

M/mL

WHO ref: ≥ 16 M/mL

M/mL

WHO ref: < 1 M/mL

%

WHO ref: ≥ 42%

%

WHO ref: ≥ 30%

%

WHO ref: ≥ 4%

Total Count and TMSC are calculated automatically from Volume × Concentration.

What this calculator analyses

WHO 2021 Standards

Compares each parameter against the latest WHO reference values for fertile men.

Derived Metrics

Automatically calculates Total Count and TMSC (Total Motile Sperm Count) from your values.

Clinical Conditions

Identifies standard andrological conditions (Oligozoospermia, Asthenozoospermia etc.) from your results.

Get expert support

Speak to our male fertility specialists about your results.

Our andrologists and fertility team can review your full semen analysis, investigate the cause, and recommend the most effective treatment pathway.

“

My semen report said 'abnormal' and I didn't even know what OAT syndrome meant. Entering my numbers into this tool told me exactly which parameters were affected — and the next steps helped me ask the right questions at my andrologist appointment. We did ICSI and now have twins.

— Patient at Bavishi Fertility Institute

Who Should Use This Calculator?

Just received your semen report

Make sense of your results before — or as preparation for — your specialist appointment.

Repeating after lifestyle changes

Track improvement across reports after making dietary or lifestyle changes over 3–6 months.

Couples investigating infertility

Male factor affects 50% of cases. This gives both partners a clearer picture from day one.

Before starting IVF/ICSI

Understand whether your TMSC supports IUI, standard IVF, or whether ICSI is the recommended path.

Monitoring a known condition

Men with varicocele, hormonal treatment, or prior infection can track parameter improvements.

Proactive male health check

No fertility concerns yet? Understanding your baseline before trying is always a positive step.

About This Tool

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